Electrostatics affects formation of Watson-Crick complex between DNA bases in monolayers of nucleolipids deposited at a gold electrode surface

Chronocoulometry was applied to determine charge in a monolayer of nucleolipid (1,2-dipalmitoyl-sn‑glycero-3-(cytidine diphosphate)) deposited at gold electrode surface. The immersion method used measure the potential zero interface ( E pzci ), which is sum on and Photon polarization infrared reflection absorption spectroscopy (PM-IRRAS) formation Watson-Crick complex between terminal cytidine moiety guanine (its complementary base) added solution. combination electrochemical spectroscopic studies allowed one demonstrate that formed when positively charged. affects not only but also orientation cytosine moiety. oriented assuming small angle with respect